While investigating the role played by de novo lipid (DNL) biosynthesis in cancer cells, we sought a medium condition that would support cell proliferation without providing any serum lipids. Here we report that a defined serum free cell culture medium condition containing insulin, transferrin and selenium (ITS) supports controlled study of transcriptional regulation of de novo fatty acid (DNFA) production and de novo cholesterol synthesis (DNCS) in melanoma cell lines. Читать дальше...

Acute bladder decentralization in hound dogs: Preliminary results of effects on hypogastric nerve electroneurograms and detrusor pressure responses to spinal root and hypogastric nerve stimulation

11.04.2019 00:00

Plos.org

by Ekta Tiwari, Mary F. Barbe, Michel A. Lemay, Danielle M. Salvadeo, Matthew W. Wood, Michael Mazzei, Luke V. Musser, Zdenka J. Delalic, Alan S. Braverman, Michael R. Ruggieri Sr.

Sensitivity for multimorbidity: The role of diagnostic uncertainty of physicians when evaluating multimorbid video case-based vignettes

11.04.2019 00:00

Plos.org

by Daniel Hausmann, Vera Kiesel, Lukas Zimmerli, Narcisa Schlatter, Amandine von Gunten, Nadine Wattinger, Thomas Rosemann

Undetectable mannose binding lectin is associated with HRCT proven bronchiectasis in rheumatoid arthritis (RA)

11.04.2019 00:00

Plos.org

by Krista Makin, Tracie Easter, Monica Kemp, Peter Kendall, Max Bulsara, Sophie Coleman, Graeme J. Carroll

An index-based method for evaluating seismic retrofitting techniques. Application to a reinforced concrete primary school in Huelva

11.04.2019 00:00

Plos.org

by María-Victoria Requena-García-Cruz, Antonio Morales-Esteban, Percy Durand-Neyra, João M. C. Estêvão

The association between alcohol consumption, cardiac biomarkers, left atrial size and re-ablation in patients with atrial fibrillation referred for catheter ablation

11.04.2019 00:00

Plos.org

by Neshro Barmano, Emmanouil Charitakis, Robert Kronstrand, Ulla Walfridsson, Jan-Erik Karlsson, Håkan Walfridsson, Fredrik H. Nystrom

Comparative analysis of homologous aminopeptidase PepN from pathogenic and non-pathogenic mycobacteria reveals divergent traits

11.04.2019 00:00

Plos.org

by Nishant Sharma, Suruchi Aggarwal, Saravanan Kumar, Rahul Sharma, Konika Choudhury, Niti Singh, Praapti Jayaswal, Renu Goel, Saima Wajid, Amit Kumar Yadav, Krishnamohan Atmakuri

Validation of the Indonesian version of the Safety Attitudes Questionnaire: A Rasch analysis

11.04.2019 00:00

Plos.org

by Evi Ningrum, Sue Evans, Sze-Ee Soh

Introduction

Safety climate, which provides a snapshot of safety culture, is rarely measured in Indonesian healthcare organisations because there are no validated surveys that can be administered in its native language, Bahasa Indonesia. The objectives of this study were to translate and linguistically adapt the Safety Attitudes Questionnaire into Bahasa Indonesia, and investigate the internal construct validity and reliability of the translated survey. Читать дальше...

Improved support vector machine classification algorithm based on adaptive feature weight updating in the Hadoop cluster environment

11.04.2019 00:00

Plos.org

by Jianfang Cao, Min Wang, Yanfei Li, Qi Zhang

An image classification algorithm based on adaptive feature weight updating is proposed to address the low classification accuracy of the current single-feature classification algorithms and simple multifeature fusion algorithms. The MapReduce parallel programming model on the Hadoop platform is used to perform an adaptive fusion of hue, local binary pattern (LBP) and scale-invariant feature transform (SIFT) features extracted from images to derive optimal combinations of weights. Читать дальше...

Crystal structure of the SH3 domain of human Lyn non-receptor tyrosine kinase

11.04.2019 00:00

Plos.org

by Sandra Berndt, Vsevolod V. Gurevich, T. M. Iverson

Lyn kinase (Lck/Yes related novel protein tyrosine kinase) belongs to the family of Src-related non-receptor tyrosine kinases. Consistent with physiological roles in cell growth and proliferation, aberrant function of Lyn is associated with various forms of cancer, including leukemia, breast cancer and melanoma. Here, we determine a 1.3 Å resolution crystal structure of the polyproline-binding SH3 regulatory domain of human Lyn kinase, which adopts a five-stranded β-barrel fold. Читать дальше...

Premedication practices for delivery room intubations in premature infants in France: Results from the EPIPAGE 2 cohort study

11.04.2019 00:00

Plos.org

by Elizabeth Walter-Nicolet, Emilie Courtois, Christophe Milesi, Pierre-Yves Ancel, Alain Beuchée, Pierre Tourneux, Valérie Benhammou, Ricardo Carbajal, Xavier Durrmeyer

A discriminatory test for the wheat B and G genomes reveals misclassified accessions of Triticum timopheevii and Triticum turgidum

11.04.2019 00:00

Plos.org

by Beata I. Czajkowska, Hugo R. Oliveira, Terence A. Brown

The tetraploid wheat species Triticum turgidum and Triticum timopheevii are morphologically similar, and misidentification of material collected from the wild is possible. We compared published sequences for the Ppd-A1, Ppd-B1 and Ppd-G1 genes from multiple accessions of T. turgidum and T. timopheevii and devised a set of four polymerase chain reactions (PCRs), two specific for Ppd-B1 and two for Ppd-G1. We used these PCRs with 51 accessions of T. Читать дальше...

Matrix modification for enhancing the transport properties of the human cartilage endplate to improve disc nutrition

11.04.2019 00:00

Plos.org

by Aaron Dolor, Sara L. Sampson, Ann A. Lazar, Jeffrey C. Lotz, Francis C. Szoka, Aaron J. Fields

Grizzly bear response to fine spatial and temporal scale spring snow cover in Western Alberta

11.04.2019 00:00

Plos.org

by Ethan E. Berman, Nicholas C. Coops, Sean P. Kearney, Gordon B. Stenhouse

Association of some Campylobacter jejuni with Pseudomonas aeruginosa biofilms increases attachment under conditions mimicking those in the environment

11.04.2019 00:00

Plos.org

by Amy Huei Teen Teh, Sui Mae Lee, Gary A. Dykes

Campylobacter jejuni is a microaerophilic bacterial species which is a major food-borne pathogen worldwide. Attachment and biofilm formation have been suggested to contribute to the survival of this fastidious bacteria in the environment. In this study the attachment of three C. jejuni strains (C. jejuni strains 2868 and 2871 isolated from poultry and ATCC 33291) to different abiotic surfaces (stainless steel, glass and polystyrene) alone or with Pseudomonas aeruginosa biofilms on them... Читать дальше...

Identification of ChIP-seq and RIME grade antibodies for Estrogen Receptor alpha

11.04.2019 00:00

Plos.org

by Silvia-E. Glont, Evangelia K. Papachristou, Ashley Sawle, Kelly A. Holmes, Jason S. Carroll, Rasmus Siersbaek

Correction: Impaired hepatic mitochondrial function during early lactation in dairy cows: Association with protein lysine acetylation

11.04.2019 00:00

Plos.org

by The PLOS ONE Staff

Новости России

Москва

Адаптация сценария. Адаптация сценария под полнометражный фильм.

Structural and catalytic insights into HoLaMa, a derivative of Klenow DNA polymerase lacking the proofreading domain

11.04.2019 00:00

Plos.org

by Michael Kovermann, Alessandra Stefan, Anna Castaldo, Sara Caramia, Alejandro Hochkoeppler

Correction: Imprinting methylation in SNRPN and MEST1 in adult blood predicts cognitive ability

11.04.2019 00:00

Plos.org

by Marlene Lorgen-Ritchie, Alison D. Murray, Anne C. Ferguson-Smith, Marcus Richards, Graham W. Horgan, Louise H. Phillips, Gwen Hoad, Ishbel Gall, Kristina Harrison, Geraldine McNeill, Mitsuteru Ito, Paul Haggarty

Correction: The distributional impact of a green payment policy for organic fruit

11.04.2019 00:00

Plos.org

by Erik Nelson, John Fitzgerald, Nathan Tefft

Correction: Does it fit?—Trainability of affordance judgments in young and older adults

11.04.2019 00:00

Plos.org

by Lisa Finkel, Simone Engler, Jennifer Randerath

Whole cell matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for identification of Leptospira spp. in Thailand and Lao PDR

11.04.2019 00:00

Plos.org

by Piengchan Sonthayanon, Janthima Jaresitthikunchai, Suthee Mangmee, Tipparat Thiangtrongjit, Vanaporn Wuthiekanun, Premjit Amornchai, Paul Newton, Rattanaphone Phetsouvanh, Nicholas PJ Day, Sittiruk Roytrakul

Efficacy of China-made praziquantel for treatment of Schistosomiasis haematobium in Africa: A randomized controlled trial

11.04.2019 00:00

Plos.org

by Xin-Yao Wang, Jian He, Saleh Juma, Fatma Kabole, Jia-gang Guo, Jian-Rong Dai, Wei Li, Kun Yang

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