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Новости за 09.07.2020

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Advantages of an easy-to-use DNA extraction method for minimal-destructive analysis of collection specimens

Plos.org 
En

by Franziska Patzold, Alberto Zilli, Anna K. Hundsdoerfer

Here we present and justify an approach for minimal-destructive DNA extraction from historic insect specimens for next generation sequencing applications. An increasing number of studies use insects from museum collections for biodiversity research. However, the availability of specimens for molecular analyses has been limited by the degraded nature of the DNA gained from century-old museum material and the consumptive nature of most DNA extraction procedures. Читать дальше...

A southern African archaeological database of organic containers and materials, 800 cal BC to cal AD 1500: Possible implications for the transition from foraging to livestock-keeping

Plos.org 
En

by Faye Lander, Thembi Russell

Although evidence of organic materials has consistently been reported in the archaeology of southern Africa little attention has been given to how this evidence, so slight in comparison to pottery and lithics, might be used to understand the transition from foraging to livestock-keeping in southern African Archaeology. We have compiled a geo-referenced, radiocarbon database of these organic, material culture remains, with particular reference to containers made of ostrich eggshell... Читать дальше...

A mini ROV-based method for recovering marine instruments at depth

Plos.org 
En

by Joseph H. Tarnecki, William F. Patterson III

Instruments are often deployed at depth for weeks to years for a variety of marine applications. In many cases, divers can be deployed to retrieve instruments, but divers are constrained by depth limitations and safety concerns. Acoustic release technology can also be employed but can add considerable expense and acoustic releases will at times fail. Here, we report a simple method that utilizes a commercially available mooring hook integrated... Читать дальше...



Purification and characterization of a novel medium-chain ribitol dehydrogenase from a lichen-associated bacterium Sphingomonas sp.

Plos.org 
En

by Kiet N. Tran, Nhung Pham, Sei-Heon Jang, ChangWoo Lee

Sugar alcohols (polyols) are abundant carbohydrates in lichen-forming algae and transported to other lichen symbionts, fungi, and bacteria. Particularly, ribitol is an abundant polyol in the lichen Cetraria sp. Polyols have important physiological roles in lichen symbiosis, but polyol utilization in lichen-associated bacteria has been largely unreported. Herein, we purified and characterized a novel ribitol dehydrogenase (RDH) from... Читать дальше...

Effect of thermal ballast loading on temperature stability of domestic refrigerators used for vaccine storage

Plos.org 
En

by Michal Chojnacky, Alexandra L. Rodriguez

Vaccine temperature control failures represent a significant public and private healthcare cost. Vaccines damaged by excessive heat or freezing lose their effectiveness, putting public health at risk. Some vaccine administration programs recommend placing water bottles inside domestic refrigerators used for vaccine storage as a thermal ballast, to mitigate temperature excursion risks. However, the effect of variable thermal ballast loading on refrigerator... Читать дальше...

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