Kinetic analysis of hemoglobin detergency by probability density functional method

by Miyako Oya, Yosuke Taniguchi, Naoaki Fujimura, Karen Miyamoto, Masaru Oya

In this study, washing tests were performed using samples prepared by contaminating fabrics with hemoglobin, and a kinetic analysis was conducted based the probability density functional method, which expresses the cleaning power using two parameters σ_rl (related to the cleaning mechanism) and μ_rl (related to the level of cleaning power). This method allows for the processing of uncertainties specific to protein washing under the assumption that the soil adhesion and detergency are in accordance with a normal distribution. A certain amount of hemoglobin solution was soaked in a cloth, dried, and steam-treated, and then used as a sample for a cleaning test. Two parameters σ_rl and μ_rl were calculated based on the detergency (%) after 5 min, 10 min, 15 min, and 20 min of washing with respect to different pH and temperature levels, and different sodium dodecyl sulfate (SDS) concentration and temperature levels. Based on the results, the value of σ_rl indicated that the hemoglobin was removed by the dissolving action. In addition, μ_rl increased in accordance with an increase in the pH, SDS concentration, and temperature. With respect to μ_rl, the relationship of ΔX + ΔY = Δ(X+Y) was observed in several cases, where ΔX represents the effect of the pH or SDS concentration, ΔY is the temperature effect, and Δ(X+Y) is the combined effect. Therefore, there may be an additive relationship between the pH and temperature effects, and the SDS concentration and temperature effects.