by Vitor Hugo Simões Miranda, Talita Rocha Gomes, Dirli Emerick Eller, Lorena de Cássia Neres Ferraz, Ana Thereza Chaves, Kelly Alves Bicalho, Carlos Eduardo Calzavara Silva, Alexander Birbrair, Marcelo Antônio Pascoal Xavier, Alfredo Miranda de Goes, Rodrigo Corrêa-Oliveira, Érica Alessandra Rocha Alves, Adriana Bozzi
Background
In view of the potential immunosuppressive and regenerative properties of mesenchymal stem cells (MSC), we investigated whether transplantation of adipose tissue-derived stem cells (ASC) could be used to control the granulomatous reaction in the liver of mice infected with Schistosoma mansoni after Praziquantel (PZQ) treatment.
Methodology/Principal findings
C57BL/6 mice infected with S. mansoni were treated with PZQ and transplanted intravenously with ASC from uninfected mice. Liver morpho-physiological and immunological analyses were performed. The combined PZQ/ASC therapy significantly reduced the volume of hepatic granulomas, as well as liver damage as measured by ALT levels. We also observed that ASC accelerated the progression of the granulomatous inflammation to the advanced/curative phase. The faster healing interfered with the expression of CD28 and CTLA-4 molecules in CD4+ T lymphocytes, and the levels of IL-10 and IL-17 cytokines, mainly in the livers of PZQ/ASC-treated mice.
Conclusions
Our results show that ASC therapy after PZQ treatment results in smaller granulomas with little tissue damage, suggesting the potential of ASC for the development of novel therapeutic approaches to minimize hepatic lesions as well as a granulomatous reaction following S. mansoni infection. Further studies using the chronic model of schistosomiasis are required to corroborate the therapeutic use of ASC for schistosomiasis.
Model viewer forensics reveal that Elden Ring: Shadow of the Erdtree's Dancing Lion boss is actually two little guys piloting it around
The latest friendship-ruining co-op game on Steam is a punishing platformer where you're chained to your pals, and it's about to crack 100,000 concurrent players
'Maybe this new Stardew Valley-like game is pretty good' I said to myself after blearily noticing I'd played it until 2 in the morning
Dustborn let me smash fascists and flirt with my situationship on a road trip across America
Два пассажира пропали после аварии с поездом Воркута — Новороссийск
Преимущества карты строек жилых и промышленных объектов в России
Филиал № 4 ОСФР по Москве и Московской области информирует:
Более 12 тысяч жителей Москвы и Московской области получают повышенную пенсию за работу в сельском хозяйстве
В Узбекистане пожаловались на задержку граждан на въезде в Россию