BoReMi: Bokeh-based jupyter-interface for registering spatio-molecular data to related microscopy images

by Jaspreet Ishar, Yee Man Tam, Simon Mages, Johanna Klughammer

Spatio-molecular data and microscopy images provide complementary information, essential to study structure and function of spatially organised multicellular systems such as healthy or diseased tissues. However, aligning these two types of data can be challenging due to distortions and differences in resolution, orientation, and position. Manual registration is tedious but may be necessary for challenging samples as well as for the generation of ground-truth data sets that enable benchmarking of existing and emerging automated alignment tools. To make the process of manual registration more convenient, efficient, and integrated, we created BoReMi, a python-based, Jupyter-integrated, visual tool that offers all the relevant functionalities for aligning and registering spatio-molecular data and associated microscopy images. We showcase BoReMi’s utility using publicly available data and images and make BoReMi as well as an interactive demo available on GitHub.