Evaluation of Monascus purpureus fermentation in dairy sludge-based medium for enhanced production of vibrant red pigment with minimal citrinin content

by Samira Moradi, Seyed Ali Mortazavi

This study aimed to explore the production of red pigment from Monascus purpureus in waste culture medium and its potential health benefits. Subsequently, the M. purpureus cultivated in a medium containing dairy sludge as waste, the extracted pigment was purified, and subjected to various analyses, including liquid chromatography mass spectrometry (LCMS) and nuclear magnetic resonance (NMR) to verify its purity, high-pressure liquid chromatography (HPLC) to measure the citrinin levels, microbial, and antioxidant activity. Finally, fermentation was conducted in a batch system using a fermenter. M. purpureus was grown in a medium composed of dairy sludge, monosodium glutamate, and glucose, resulting in a biomass yield of 26.15 g/L. After extraction and purification, the sample yielded 4.85 g of dry color. Analysis confirmed the purity of the pigment by LCMS and NMR and revealed low citrinin levels by HPLC. In the fermenter, the sample obtained from enriched culture conditions displayed the highest concentration of monascorubramine, maximum specific growth rate of 0.029/1/h, a cell yield (Y_x/s) of 0.29 g/g, and a production efficiency of 65% for M. purpureus. The produced pigment sample showed potential for use in the food industry due to its low citrinin content and high concentration of red pigment.