Although stimulated-emission depletion (STED) microscopy was a true breakthrough for observing the morphology of live neurons at higher resolution, there is still room for improvement. In a recent study published in Neurophotonics, a team of scientists led by Dr. U. Valentin Nägerl from Université de Bordeaux developed a simple yet effective calibration method that allows for more precise STED imaging at higher tissue depths.
