Добавить новость
ru24.net
Все новости
Февраль
2024

Synthesis, insertion, and characterization of SARS-CoV-2 membrane protein within lipid bilayers | Science Advances

0

Abstract

Throughout history, coronaviruses have posed challenges to both public health and the global economy; nevertheless, methods to combat them remain rudimentary, primarily due to the absence of experiments to understand the function of various viral components. Among these, membrane (M) proteins are one of the most elusive because of their small size and challenges with expression. Here, we report the development of an expression system to produce tens to hundreds of milligrams of M protein per liter of Escherichia coli culture. These large yields render many previously inaccessible structural and biophysical experiments feasible. Using cryo–electron microscopy and atomic force microscopy, we image and characterize individual membrane-incorporated M protein dimers and discover membrane thinning in the vicinity, which we validated with molecular dynamics simulations. Our results suggest that the resulting line tension, along with predicted induction of local membrane curvature, could ultimately drive viral assembly and budding.



Moscow.media
Частные объявления сегодня





Rss.plus




Спорт в России и мире

Новости спорта


Новости тенниса
WTA

Полина Кудерметова вышла во второй круг турнира WTA в Брисбене






Семейные новогодние традиции

Сергунина пригласила москвичей и туристов посетить «Путешествие в Рождество» на каникулах

Москва утвердила 18 новых проектов метро: улучшение для миллиона жителей

Сегодня голос Марии Захаровой вспоминает неожиданный уход Бориса Ельцина